Salmonella Product Support
Useful Links
White Papers
- Pet food white paper
- qPCR comparative analysis (turkey spleen samples)
- Broiler study (ceca samples, vaccinated vs non-vaccinated)
- Culture comparative analysis (poultry environmental samples)
Salmonella FAQ
How do I prepare mBPW?
Make sure you prepare your mBPW according to the protocol outlined in the Product Insert included with the DNAble Salmonella Media Supplement kit.
Why do I need to equilibrate the mBPW to 37°C before use?
Having the mBPW at 37°C optimizes the conditions for the growth of Salmonella.
Important Steps for running the DNAble Salmonella Test:
To obtain the best results, follow the protocol outlined in the Product Insert.
Which DNAble Salmonella Extraction Set should I use?
Contact Technical Support for help with determining the appropriate extraction set for your testing.
Ext Set 1 is NPIP certified
Ext Set 2 is AOAC certified
What matrices are approved for the Salmonella assay?
The validated matrices are listed here. Please contact Technical Support for more information on limited testing done on additional matrices.
What are the most important steps for running the DNAble Salmonella test?
As with any assay, it is important to strictly follow the protocol outlined in the Product Insert.
What equipment is needed for running the Salmonella test?
Contact Tech Support for a list of equipment needed to set up your lab for DNAble testing
Is the test kit certified?
The DNAble Salmonella test kit has NPIP and AOAC certification.
What should I do if contamination is suspected?
Contact Tech Support :
TECHNICAL SUPPORT
Call Toll-free US: 1-866-408-4597 ext.2 or (207) 797-0300 ext.2
Does EnviroLogix provide a negative control for the DNAble Salmonella assay?
We do not have a designated negative control. You may use either the MB2 or MB3 from the DNAble Extraction Set 1 or 2 as a negative control. See the instructions on Page 1 of the Positive Control protocol.
What steps should be taken when preparing, storing and using the re-suspended control?
Before opening the tube containing the dried down control, lift the tube up to the light to make sure the pellet is at the bottom of the tube. You should see a triangular-shaped drop of material at the bottom of the tube. If the dried down material is missing or has detached from the bottom of the tube, call Technical Support: Toll-free US 1-866-408-4597 ext.2 or (207) 797-0300 ext.2
Be sure to use the DNAse-free water to re-suspend the control. Add exactly 65 µL of the DNAse-free water with a calibrated pipette.
It is recommended that the following pipette tips (or equivalent) be used for re-suspending the positive control:USA Scientific Catalog # 1120-8810. TipOne 1-200 ul aerosol barrier filter pipet tips, sterile, 10 racks of 96 tips (960 tips)
Filter barrier protects pipettors and samples from contamination
50, 100, and 200 µl graduations
Hydrophobic filters contain no additives and will not absorb samples
Certified free of detectable RNAse, DNAse, DNA, and pyrogens
Sterilized
Yellow color-coded tip wafers
Briefly vortex the re-suspended control to obtain a uniform suspension. Excessive vortexing may compromise the integrity of the control.
Record the date the control was re-suspended on the tube. Make sure the cap is secured tightly before placing the control back in the refrigerator.
Do not store the re-suspended control in its original pouch, as the pouch contains a packet of desiccant that may compromise the performance of the control.
Before using a re-suspended control that has been refrigerated, allow the control to warm to room temperature. Make sure all the liquid is at the bottom of the tube by tapping/flicking the tube. Briefly vortex the control to mix.
What is the stability of the control?
The tubes of dried down control and DNAse-free water are stable at 2-8°C up to the expiration date listed on the kit. The re-suspended control is stable for 2 weeks when stored at 2-8°C. Always store the Salmonella Positive Control Kit components at 2-8°C when not in use.
What are the components in the Positive Control Accessory Kit?
The kit comes with 2 tubes of dried down positive control and one tube of DNAse-free water for re-suspending the controls.
Does the Salmonella Positive Control contain any infectious material?
No.
