Rapid Point-of-Need Molecular Detection of Roundup Ready^® 2 Positive Soybeans Using DNAble^® Technology

• An EnviroLogix DNAble assay for cp4 epsps was run in the field and challenged with genetically modified RR2 soybeans
• The assay performed with 100% sensitivity to the Roundup Ready 2 Yield^® trait at 0.5%
• The assay performed with >99% accuracy for non-RR2 soy GMO traits
• The assay is robust and easy to use. It produces results that correlate with PCR in under 30 minutes total time

DNAble is a simple, rapid isothermal nucleic acid (molecular) amplification method with results similar to PCR. However, unlike the more costly and time-consuming PCR, DNAble is highly effective testing crude sample extracts that can be prepared at point-of-need in under 10 minutes.

The DNAble Molecular Detection Kit for v2 cp4 epsps  (MON89788) from EnviroLogix detects a unique sequence present in MON89788 soybeans (Roundup Ready 2 Yield [RR2], Roundup Ready 2 Xtend^®). The performance of this kit in ground soybean is described below with 3^rd party validation data conducted by NSF International’s Applied Research Center (NSF).

Both internal and independent validation studies resulted in >99% accuracy from conventional ground soy and 100% sensitivity for 0.5% RR2 ground soy.

In-House: Conventional and 0.5% RR2=40 independent samples, three assay kit lots, two operators

3^rd Party: Conventional=18 sub-samples, three assay kit lots; 0.5% RR2=10 independently blended samples, 3 replicate sub-samples across three assay kit lots

 

Click here for the full White Paper on the NSF website.

Authors:

Tapley¹, S. Johnson¹, B. Schindler², J. Miller², A. Rice¹, E. Whiston¹, J. Welch¹, and B. Parker¹

¹EnviroLogix Inc, Portland, ME                       ²NSF International, Ann Arbor, MI